MALDI-imaging mass spectrometry

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry, also called MALDI-imaging, is a high-throughput label-free molecular analytical technology for spatial analysis of various samples and is one of the most promising innovative measurement technologies in biochemistry. In the last decade, MALDI-imaging has been developed tremendously and currently is a powerful and versatile tool for spatial chemical analysis of a wide range of samples, from biological and plant tissues to bio and polymer thin films. The ability to simultaneously visualize multiple molecular distributions across the surface of a sample without the need for chemical labels or antibodies is the main strength of MALDI-imaging and led to rapid incorporation of the technology into new biochemical and medical research strategies. Many research groups have reported success in using MALDI-imaging for solving the crucial challenges of, in particular, biomarker discovery, drug and metabolites imaging, and biotechnological development of new drugs, that has proven the potential of this technology.

MULTI-ARRAY electrochemiluminescence technology platform

The MULTI-ARRAY technology provides a tool for the analysis of specific biomarkers on a multi-array format, thus combining the spatial resolution achieved by MALDI with the specific protein identification by MULTI-ARRAY. On a flexible 96-well assay, this technology enables detection of a wide range of biomarkers on single or multiplex formats. The multiplex measurement is based on an electrochemiluminescence principle. This method is characterized by an increased sensitivity and a high signal to background ratio. Furthermore, it features an up to 100 -fold higher binding capacity and a high dynamic detection range (1- 10,000) which allows even for the detection of low affinity antibodies. This was impossible using similar techniques (e.g. luminex based) in the past. A very small sample volume of  20-45 µl  (e.g. cell culture supernatant or serum) is required to measure up to 10 analytes in the same well. Moreover, flexible surface coatings permit customer specific applications and combinations. The presented and offered method allows for the analysis of subclinical markers in metabolic diseases in a minimal sample volume.